EMPIAR-11069
Cryo electron microscopy micrographs of high molecular weight fractions from yeast native cell extracts [multiple data sets in MRC format]
Publication:

Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts

Schmidt L, Tueting C, Kyrilis F, Hamdi F, Semchonok DA, Hause G, Meister A, Stubbs MT, Ihling C, Shah PNM, Sinz A, Stuart DI, Kastritis PL

bioRxiv (2022)

Related EMDB entry:
Deposited:
2022-06-16
Released:
2024-01-23
Last modified:
2024-01-23
Imageset size:
4.66 TB
Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (FoilHole*.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   9,032,250,836 kilobytes
Compressed size: 5,003,097,717 kilobytes
Contains:
  • micrographs - multiframe
1. Multi-frame micrographs for Saccharomyces cerevisiae native cell extract fraction
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2728
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(3.177, 3.177)
Details:
Cryo-EM micrographs of Saccharomyces cerevisiae fractionated cell extract. Analyzed fraction is enriched in the endogenous L-A helper virus and Ribo- and Polysomes.
Micrographs were acquired at a dose of 30 Å/px and a pixel size of 3.177.
Files:
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2. Multi-frame micrographs for Saccharomyces cerevisiae native cell extract fraction
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
7813
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(3.177, 3.177)
Details:
Cryo-EM micrographs of Saccharomyces cerevisiae fractionated cell extract. Analyzed fraction is enriched in the endogenous L-A helper virus and Ribo- and Polysomes.
Micrographs were acquired at a dose of 30 Å/px and a pixel size of 3.177.
Files:
Loading...
3. Multi-frame micrographs for Saccharomyces cerevisiae native cell extract fraction
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2254
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(3.177, 3.177)
Details:
Cryo-EM micrographs of Saccharomyces cerevisiae fractionated cell extract. Analyzed fraction is enriched in the endogenous L-A helper virus and Ribo- and Polysomes.
Micrographs were acquired at a dose of 30 Å/px and a pixel size of 3.177.
Files:
Loading...
4. Multi-frame micrographs for Saccharomyces cerevisiae native cell extract fraction
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
4033
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.5678, 1.5678)
Details:
Cryo-EM micrographs of Saccharomyces cerevisiae fractionated cell extract. Analyzed fraction is enriched in the endogenous L-A helper virus and Ribo- and Polysomes.
Micrographs were acquired at a dose of 30 Å/px and a pixel size of 1.5678, allowing for reaching atomic resolution (3.8 Å) for the L-A helper virus.
Files:
Loading...
5. Multi-frame micrographs for Saccharomyces cerevisiae native cell extract fraction
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2978
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.5678, 1.5678)
Details:
Cryo-EM micrographs of Saccharomyces cerevisiae fractionated cell extract. Analyzed fraction is enriched in the endogenous L-A helper virus and Ribo- and Polysomes.
Micrographs were acquired at a dose of 30 Å/px and a pixel size of 1.5678, allowing for reaching atomic resolution (3.8 Å) for the L-A helper virus.
Files:
Loading...
Files:
Loading...
Citations

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)