EMPIAR-10952 - SARS-CoV-2 spike protein (1-up RBD) on Quantifoil grid [2403 multi-frame micrographs composed of 60 frames each in TIFF format]

EMPIAR-10952

SARS-CoV-2 spike protein (1-up RBD) on Quantifoil grid [2403 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

Epoxidized graphene grid for highly efficient high-resolution cryoEM structural analysis

Fujita J, Makino F, Asahara H, Moriguchi M, Kumano S, Anzai I, Kishikawa J, Matsuura Y, Kato T, Namba K, Inoue T

Scientific reports 13 (2023)

PMID: 36755111

DOI: 10.1038/s41598-023-29396-0

Related EMDB entry:

EMD-32161

Deposited:

2021-11-12

Released:

2023-02-28

Last modified:

2023-02-28

Imageset size:

594.82 GB

Imageset DOI:

10.6019/EMPIAR-10952

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of SARS-CoV-2 spike protein (1-up RBD) on Quantifoil grid

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2403

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.87, 0.87)

Details:

Unaligned and not gain corrected 60 frame movies with an accumulated exposure of 60 electrons per squared Angstrom. The gain reference was generated from a part of the dataset with "relion_estimate_gain" program in RELION 3.1.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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