EMPIAR-10952 - SARS-CoV-2 spike protein (1-up RBD) on Quantifoil grid [2403 multi-frame micrographs composed of 60 frames each in TIFF format]

EMPIAR-10952

SARS-CoV-2 spike protein (1-up RBD) on Quantifoil grid [2403 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

Epoxidized graphene grid for highly efficient high-resolution cryoEM structural analysis

Fujita J, Makino F, Asahara H, Moriguchi M, Kumano S, Anzai I, Kishikawa J, Matsuura Y, Kato T, Namba K, Inoue T

Scientific reports 13 (2023)

PMID: 36755111

DOI: 10.1038/s41598-023-29396-0

Related EMDB entry:

EMD-32161

Deposited:

2021-11-12

Released:

2023-02-28

Last modified:

2023-02-28

Imageset size:

594.82 GB

Imageset DOI:

10.6019/EMPIAR-10952

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of SARS-CoV-2 spike protein (1-up RBD) on Quantifoil grid

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2403

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.87, 0.87)

Details:

Unaligned and not gain corrected 60 frame movies with an accumulated exposure of 60 electrons per squared Angstrom. The gain reference was generated from a part of the dataset with "relion_estimate_gain" program in RELION 3.1.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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