EMPIAR-10694 - In situ cryo-electron tomogram of a pyrenoid inside a Chlamydomonas reinhardtii cell (tilt series) [1 tilt series in MRC format]

EMPIAR-10694

In situ cryo-electron tomogram of a pyrenoid inside a Chlamydomonas reinhardtii cell (tilt series) [1 tilt series in MRC format]

Publication:

The Eukaryotic CO2-Concentrating Organelle Is Liquid-like and Exhibits Dynamic Reorganization

Freeman Rosenzweig ES, Xu B, Kuhn Cuellar L, Martinez-Sanchez A, Schaffer M, Strauss M, Cartwright HN, Ronceray P, Plitzko JM, Förster F, Wingreen NS, Engel BD, Mackinder LCM, Jonikas MC

Cell 171 (2017) 148-162.e19

PMID: 28938114

DOI: 10.1016/j.cell.2017.08.008

Related EMDB entry:

EMD-12749

Deposited:

2021-04-13

Released:

2021-04-14

Last modified:

2021-04-14

Imageset size:

5.75 GB

Imageset DOI:

10.6019/EMPIAR-10694

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Phase-flipped and aligned tilt series of a pyrenoid inside a Chlamydomonas reinhardtii cell

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3712, 3712)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(3.42, 3.42)

Details:

L3Tomo3.st is the original unbinned tilt series after motion correction and dose-weighting of individual tilts.
L3Tomo3.st is the aligned and phase-flipped unbinned tilt series for tomogram reconstruction.
L3Tomo3_bin4.rec is the bin4 tomogram for IMOD consistency.
Other files are accessory for tomogram reconstruction and subtomogram extraction. See README.txt for details.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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