EMPIAR-10612 - High-resolution SARS-CoV-2 ORF3a dimer in an MSP1E3D1 lipid nanodisc [multiple data sets in EER format]

EMPIAR-10612

High-resolution SARS-CoV-2 ORF3a dimer in an MSP1E3D1 lipid nanodisc [multiple data sets in EER format]

Publication:

Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs

Kern DM, Sorum B, Hoel CM, Sridharan S, Remis JP, Toso DB, Brohawn SG

bioRxiv (2020)

DOI: 10.1101/2020.06.17.156554

Related EMDB entry:

EMD-22898

Deposited:

2020-11-28

Released:

2021-02-05

Last modified:

2023-02-20

Imageset size:

3.16 TB

Imageset DOI:

10.6019/EMPIAR-10612

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned movies in EER format of SARS-CoV-2 3a in MSP1E3D1 lipid nanodiscs - 1379 frames

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

789

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.3635, 0.3635)

Details:

EER-format movies are in folders titled by total frame number. We recommend merging 30 frames for one dose fraction which gives 45 frame movies. The dose per frame is 0.0345 e/A2 (or 1.035e/A2 per suggested fraction). The gain reference image is in the movies folder. The super-resolution pixel size is 0.3635 A for a physical pixel size of 0.727 A. A cryosparc non-uniform refinement folder (containing polished particles) is also included.

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2. Unaligned movies in EER format of SARS-CoV-2 3a in MSP1E3D1 lipid nanodiscs - 1449 frames

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

4809

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.3635, 0.3635)

Details:

EER-format movies are in folders titled by total frame number. We recommend merging 30 frames for one dose fraction which gives 48 frame movies. The dose per frame is 0.0345 e/A2 (or 1.035e/A2 per suggested fraction). The gain reference image is in the movies folder. The super-resolution pixel size is 0.3635 A for a physical pixel size of 0.727 A. A cryosparc non-uniform refinement folder (containing polished particles) is also included.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

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