EMPIAR-10612
High-resolution SARS-CoV-2 ORF3a dimer in an MSP1E3D1 lipid nanodisc [multiple data sets in EER format]
Publication:

Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs

Kern DM, Sorum B, Hoel CM, Sridharan S, Remis JP, Toso DB, Brohawn SG

bioRxiv (2020)

Related EMDB entry:
Deposited:
2020-11-28
Released:
2021-02-05
Last modified:
2023-02-20
Imageset size:
3.16 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned movies in EER format of SARS-CoV-2 3a in MSP1E3D1 lipid nanodiscs - 1379 frames
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
789
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.3635, 0.3635)
Details:
EER-format movies are in folders titled by total frame number. We recommend merging 30 frames for one dose fraction which gives 45 frame movies. The dose per frame is 0.0345 e/A2 (or 1.035e/A2 per suggested fraction). The gain reference image is in the movies folder. The super-resolution pixel size is 0.3635 A for a physical pixel size of 0.727 A. A cryosparc non-uniform refinement folder (containing polished particles) is also included.
Files:
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2. Unaligned movies in EER format of SARS-CoV-2 3a in MSP1E3D1 lipid nanodiscs - 1449 frames
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
4809
Image size:
(4096, 4096)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.3635, 0.3635)
Details:
EER-format movies are in folders titled by total frame number. We recommend merging 30 frames for one dose fraction which gives 48 frame movies. The dose per frame is 0.0345 e/A2 (or 1.035e/A2 per suggested fraction). The gain reference image is in the movies folder. The super-resolution pixel size is 0.3635 A for a physical pixel size of 0.727 A. A cryosparc non-uniform refinement folder (containing polished particles) is also included.
Files:
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