EMPIAR-10495
Structure and assembly of ESCRT-III helical Vps24 filaments [1320 micrographs in MRC format]
Publication:

Structure and assembly of ESCRT-III helical Vps24 filaments

Huber ST, Mostafavi S, Mortensen SA, Sachse C

Science Advances 6 (2020)

Related EMDB entry:
Deposited:
2020-07-27
Released:
2020-11-18
Last modified:
2020-11-18
Imageset size:
70.02 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. Aligned micrographs of Vps24 helical filaments
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1320
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.04, 1.04)
Details:
Selection of 1320 K2 micrographs based on specimen coverage, ice quality, and minimal
drift. The same micrographs were used in the paper. Movies were motion-corrected and dose-weighted using MotionCor2. 40 e-/A2 cumulative exposure, 0.75 um to 3 um defocus range. 4 images per hole were taken on Quantifoil R2/1 grids.
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