EMPIAR-10495 - Structure and assembly of ESCRT-III helical Vps24 filaments [1320 micrographs in MRC format]

EMPIAR-10495

Structure and assembly of ESCRT-III helical Vps24 filaments [1320 micrographs in MRC format]

Publication:

Structure and assembly of ESCRT-III helical Vps24 filaments

Huber ST, Mostafavi S, Mortensen SA, Sachse C

Science Advances 6 (2020)

DOI: 10.1126/sciadv.aba4897

Related EMDB entry:

EMD-11212

Deposited:

2020-07-27

Released:

2020-11-18

Last modified:

2020-11-18

Imageset size:

70.02 GB

Imageset DOI:

10.6019/EMPIAR-10495

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Aligned micrographs of Vps24 helical filaments

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1320

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.04, 1.04)

Details:

Selection of 1320 K2 micrographs based on specimen coverage, ice quality, and minimal
drift. The same micrographs were used in the paper. Movies were motion-corrected and dose-weighted using MotionCor2. 40 e-/A2 cumulative exposure, 0.75 um to 3 um defocus range. 4 images per hole were taken on Quantifoil R2/1 grids.

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