EMPIAR-10469 - Single particle cryo EM dataset of SARS-CoV-2 Spike protein in uncleavable form, which generated a 2.6Å reconstruction of the closed trimer. [9210 multi-frame micrographs composed of 30 frames each in MRC format]

EMPIAR-10469

Single particle cryo EM dataset of SARS-CoV-2 Spike protein in uncleavable form, which generated a 2.6Å reconstruction of the closed trimer. [9210 multi-frame micrographs composed of 30 frames each in MRC format]

Publication:

SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects

Wrobel AG, Benton DJ, Xu P, Roustan C, Martin SR, Rosenthal PB, Skehel JJ, Gamblin SJ

Nature Structural and Molecular Biology (2020)

PMID: 32647346

DOI: 10.1038/s41594-020-0468-7

Related EMDB entry:

EMD-11203

Deposited:

2020-06-24

Released:

2020-07-21

Last modified:

2020-07-21

Imageset size:

8.43 TB

Imageset DOI:

10.6019/EMPIAR-10469

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned Movies of uncleavable SARS-CoV-2 Spike protein ectodomain

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

9210

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.087, 1.087)

Details:

Movies collected of uncleavable SARS-CoV-2 S protein. Movies collected on a Titan Krios at 300kV on a Falcon 3 detector in electron counting mode. Movies are 30 frames with 1.13 e/Å^2/frame with a total dose of 33.9 e/Å^2.

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Citations

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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