EMPIAR-10469
Single particle cryo EM dataset of SARS-CoV-2 Spike protein in uncleavable form, which generated a 2.6Å reconstruction of the closed trimer. [9210 multi-frame micrographs composed of 30 frames each in MRC format]
Publication:

SARS-CoV-2 and bat RaTG13 spike glycoprotein structures inform on virus evolution and furin-cleavage effects

Wrobel AG, Benton DJ, Xu P, Roustan C, Martin SR, Rosenthal PB, Skehel JJ, Gamblin SJ

Nature Structural and Molecular Biology (2020)

PMID: 32647346

Related EMDB entry:
Deposited:
2020-06-24
Released:
2020-07-21
Last modified:
2020-07-21
Imageset size:
8.43 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned Movies of uncleavable SARS-CoV-2 Spike protein ectodomain
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
9210
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.087, 1.087)
Details:
Movies collected of uncleavable SARS-CoV-2 S protein. Movies collected on a Titan Krios at 300kV on a Falcon 3 detector in electron counting mode. Movies are 30 frames with 1.13 e/Å^2/frame with a total dose of 33.9 e/Å^2.
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