EMPIAR-10409 - Structure of replicating SARS-CoV-2 polymerase [multiple data sets in TIFF and MRCS formats]

EMPIAR-10409

Structure of replicating SARS-CoV-2 polymerase [multiple data sets in TIFF and MRCS formats]

Publication:

Structure of replicating SARS-CoV-2 polymerase

Hillen HS, Kokic G, Farnung L, Dienemann C, Tegunov D, Cramer P

Nature (2020)

PMID: 32438371

DOI: 10.1038/s41586-020-2368-8

Related EMDB entry:

EMD-11007

Deposited:

2020-05-06

Released:

2020-05-13

Last modified:

2023-01-23

Imageset size:

2.96 TB

Imageset DOI:

10.6019/EMPIAR-10409

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA (part 1)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3092

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.834, 0.834)

Details:

Movies are from K3 (non-superres) and uncorrected (gain can be found in ./gain subfolders). Each LZW compressed.tif movie is accompanied with its respective .xml metadata file generated by Warp. Movies contain 80 frames collected over 2.2 seconds with a total dose of 60 e-/A2. Data was collected at 30 degree stage tilt. Particle locations were picked with Warp (locations are stored in ./matching subfolder). Micrograph thumbnails can be found in ./thumbnails subfolders.

Files:

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2. Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA (part 2)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5076

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.834, 0.834)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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3. Warp picked and extracted particles

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

1385860

Image size:

(240, 240)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.834, 0.834)

Details:

Particles were picked and extracted using Warp.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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4. Reextraced particles at 1.3 A/px (for Map2 and Map3)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

415690

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3, 1.3)

Details:

Particles from Map1 were reextraced using Relion 3 at 1.3 A/px. Refer to ED Figure 1 of the paper.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Refined particles (Map 1)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

417736

Image size:

(240, 240)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.834, 0.834)

Details:

Refined particles for Map1 (refer to ED Figure 1 of the paper).

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

6. Refined particles (Map 2)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

172369

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3, 1.3)

Details:

Refined particles for Map2 (refer to ED Figure 1 of the paper).

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

7. Refined particles (Map 3)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

172369

Image size:

(256, 256)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.3, 1.3)

Details:

Refined particles for Map3 (refer to ED Figure 1 of the paper).

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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