EMPIAR-10409
Structure of replicating SARS-CoV-2 polymerase [multiple data sets in TIFF and MRCS formats]
Publication:

Structure of replicating SARS-CoV-2 polymerase

Hillen HS, Kokic G, Farnung L, Dienemann C, Tegunov D, Cramer P

Nature (2020)

PMID: 32438371

Related EMDB entry:
Deposited:
2020-05-06
Released:
2020-05-13
Last modified:
2023-01-23
Imageset size:
2.96 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA (part 1)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3092
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.834, 0.834)
Details:
Movies are from K3 (non-superres) and uncorrected (gain can be found in ./gain subfolders). Each LZW compressed.tif movie is accompanied with its respective .xml metadata file generated by Warp. Movies contain 80 frames collected over 2.2 seconds with a total dose of 60 e-/A2. Data was collected at 30 degree stage tilt. Particle locations were picked with Warp (locations are stored in ./matching subfolder). Micrograph thumbnails can be found in ./thumbnails subfolders.
Files:
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2. Unaligned TIF movies of SARS-CoV2 RdRp in complex with nsp7, nsp8 and RNA (part 2)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5076
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.834, 0.834)
Details:
Movies are from K3 (non-superres) and uncorrected (gain can be found in ./gain subfolders). Each LZW compressed.tif movie is accompanied with its respective .xml metadata file generated by Warp. Movies contain 80 frames collected over 2.2 seconds with a total dose of 60 e-/A2. Data was collected at 30 degree stage tilt. Particle locations were picked with Warp (locations are stored in ./matching subfolder). Micrograph thumbnails can be found in ./thumbnails subfolders.
Files:
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3. Warp picked and extracted particles
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
1385860
Image size:
(240, 240)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.834, 0.834)
Details:
Particles were picked and extracted using Warp.
Files:
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4. Reextraced particles at 1.3 A/px (for Map2 and Map3)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
415690
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3, 1.3)
Details:
Particles from Map1 were reextraced using Relion 3 at 1.3 A/px. Refer to ED Figure 1 of the paper.
Files:
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5. Refined particles (Map 1)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
417736
Image size:
(240, 240)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.834, 0.834)
Details:
Refined particles for Map1 (refer to ED Figure 1 of the paper).
Files:
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6. Refined particles (Map 2)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
172369
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3, 1.3)
Details:
Refined particles for Map2 (refer to ED Figure 1 of the paper).
Files:
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7. Refined particles (Map 3)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
172369
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.3, 1.3)
Details:
Refined particles for Map3 (refer to ED Figure 1 of the paper).
Files:
Loading...
Files:
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