EMPIAR-10386 - Human apo ferritin frozen on TEM grid with Amorphous nickel titanium alloy supporting film [426 multi-frame micrographs composed of 32 frames each in TIFF format]

EMPIAR-10386

Human apo ferritin frozen on TEM grid with Amorphous nickel titanium alloy supporting film [426 multi-frame micrographs composed of 32 frames each in TIFF format]

Publication:

Amorphous nickel titanium alloy film: A new choice for cryo electron microscopy sample preparation

Huang X, Zhang L, Wen Z, Chen H, Li S, Ji G, Yin CC, Sun F

Progress in biophysics and molecular biology 156 (2020) 3-13

PMID: 32758492

DOI: 10.1016/j.pbiomolbio.2020.07.009

Related EMDB entry:

EMD-30084

Deposited:

2020-03-14

Released:

2021-08-13

Last modified:

2021-08-13

Imageset size:

134.54 GB

Imageset DOI:

10.6019/EMPIAR-10386

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Human apo ferritin frozen on TEM grid with Amorphous nickel titanium alloy supporting film

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

426

Image size:

(7676, 7420)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.435, 0.435)

Details:

The raw data were taken in K2 super resolution mode. There are 426 multi-frame stacks without gain correction, motion correction and distortion correction in TIFF format with LZW compression of 8bit. The gain file were also provided in data/ANTA/gain.mrc (7676*7420, 32bit). The distortion parameter were distotaion angle of 26.1, major scale of 1.019 and minor scale of 1. The total dose was 60 e/A2.
Particle positions in corresponding coordinate start file were used to extract particles from bin2 micrographs for final reconstruction.

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Citations

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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