Human apo ferritin frozen on TEM grid with Amorphous nickel titanium alloy supporting film [426 multi-frame micrographs composed of 32 frames each in TIFF format]
Publication:
Amorphous nickel titanium alloy film: A new choice for cryo electron microscopy sample preparation
Huang X, Zhang L, Wen Z, Chen H, Li S, Ji G, Yin CC, Sun F
Progress in biophysics and molecular biology156 (2020) 3-13
The raw data were taken in K2 super resolution mode. There are 426 multi-frame stacks without gain correction, motion correction and distortion correction in TIFF format with LZW compression of 8bit. The gain file were also provided in data/ANTA/gain.mrc (7676*7420, 32bit). The distortion parameter were distotaion angle of 26.1, major scale of 1.019 and minor scale of 1. The total dose was 60 e/A2. Particle positions in corresponding coordinate start file were used to extract particles from bin2 micrographs for final reconstruction.
