Processed FIB SEM images of a parasitophorous vacuole containing Toxoplasma gondii ∆CAP parasites. [1 multi-frame micrographs composed of 1 frames each in MRC format]
Publication:
Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii
Image acquisition; 5 nm voxels: xy pixel set to 5 nm, FIB mill every 5 nm. SEM: 1.5 kV with 1 nA current, 10 μs dwell time. EsB detector grid voltage: 1200 V. Ion beam milling: 30 kV, 700 pA.
Image processing; Zeiss Atlas 5.2.1.25; Gradient align Export of cropped region of interest exported as 8 bit TIF files
Fiji; Invert Gaussian blur: Sigma 0.75 Unsharp mask: Radius (Sigma) 1 pixel, Mask weight 0.6 Rotate to straighten XY (D19: no rotation) Reslice: YZ assigned to XY plane Scale to 10 nm isotropic voxels
IMOD; tif2mrc
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Archives and downloads the selected files into an uncompressed zip file.
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Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
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It is possible to download files by specifying the file list with rsync command, etc.