EMPIAR-10155 - Cryo-electron tomography of the yeast NPC [120 micrographs in MRC format]

EMPIAR-10155

Cryo-electron tomography of the yeast NPC [120 micrographs in MRC format]

Publication:

Integrative structure and functional anatomy of a nuclear pore complex

Kim SJ, Fernandez-Martinez J, Nudelman I, Shi Y, Zhang W, Raveh B, Herricks T, Slaughter BD, Hogan J, Upla P, Chemmama IE, Pellarin R, Echeverria I, Shivaraju M, Chaudhury AS, Wang J, Williams R, Unruh JR, Greenberg CH, Jacobs EY, Yu Z, Jason de la Cruz M, Mironska R, Stokes DL, Aitchison JD, Jarrold MF, Gerton JL, Ludtke SJ, Akey CW, Chait BT, Sali A, Rout MP, Hogan JA, de la Cruz MJ

Nature (2018)

PMID: 29539637

DOI: 10.1038/nature26003

Related EMDB entry:

EMD-7321

Deposited:

2018-01-18

Released:

2018-03-20

Last modified:

2018-03-20

Imageset size:

127.14 GB

Imageset DOI:

10.6019/EMPIAR-10155

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Unaligned K2 DED image tilt series of yeast NPC and associated files

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

120

Image size:

(3710, 3710)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(5.6, 5.6)

Details:

Data collected with UCSF Tomo and best fit value for pixel size is 5.3 Angstroms. Variable number of tilts in the tilt series, due to different sampling and some higher tilted images with bad drift were removed. Additional files are provided , adoc parameter files used
in IMOD Batchtomo, list files with nominal and fitted tilt angles and
fitted defocus values. Coordinate files for particles selected with
e2spt_boxer.py are also given, a box size of 300 was used for
final processing with unbinned data.

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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