EMPIAR-14064 - Cryo-EM of E. coli RNA polymerase initial transcribing complexes on T7A1 promoter [multiple data sets in TIFF format]

EMPIAR-14064

Cryo-EM of E. coli RNA polymerase initial transcribing complexes on T7A1 promoter [multiple data sets in TIFF format]

Publication:

Structural basis for multi-subunit DNA-dependent RNA polymerase catalytic activity

Mueller AU, Darst SA

Molecular cell 86 (2026) 1881-189500000.0

PMID: 42066755

DOI: 10.1016/j.molcel.2026.03.033

Related EMDB entry:

EMD-73123 EMD-73125 EMD-73126 EMD-73124 EMD-73128 EMD-73130 EMD-73122 EMD-73127 EMD-73131

Deposited:

2026-09-16

Released:

2026-10-29

Last modified:

2026-10-29

Imageset size:

15.15 TB

Imageset DOI:

10.6019/EMPIAR-14064

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of E. coli RNA polymerase on T7A1 promoter DNA with ATP, UTP, CTP - dataset 1

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1440

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.86, 0.86)

Details:

The micrographs contain unaligned raw frames. Data was collected on a 300 kV Titan Krios (ThermoFisher Scientific) equipped with a K3 camera (Gatan), a Cs corrector and a BioQuantum imaging filter (Gatan) in counting mode (pixel size 0.86 A/px). Images were recorded using SerialEM over a nominal defocus range of -0.8 μm to -2.2 μm and 20 eV energy filter slit width. Collection was performed with an electron dose of 25 electrons/px/s (at the camera) in dose-fractionation mode of 0.04 seconds over a 1.4 second exposure (35 frames) to give a total dose of 47 electrons/A^2.

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2. Unaligned multi-frame micrographs of E. coli RNA polymerase on T7A1 promoter DNA with ATP, UTP, CTP - dataset 2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

13806

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.43, 0.43)

Details:

The micrographs contain unaligned raw frames. Data was collected on a 300 kV Titan Krios (ThermoFisher Scientific) equipped with a K3 camera (Gatan), a Cs corrector and a BioQuantum imaging filter (Gatan) in super-resolution mode (nominal pixel size of 0.43 A/px). Images were recorded using SerialEM over a nominal defocus range of -0.8 μm to -2.2 μm and 20 eV energy filter slit width. Collection was performed with an electron dose of 25 electrons/px/s (at the camera) in dose-fractionation mode of 0.04 seconds over a 1.4 second exposure (35 frames) to give a total dose of 47 electrons/A^2.

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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3. Unaligned multi-frame micrographs of E. coli RNA polymerase on T7A1 promoter DNA with ATP, UTP, CTP - dataset 3

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5256

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.43, 0.43)

Details:

The micrographs contain unaligned raw frames. Data was collected on a 300 kV Titan Krios (ThermoFisher Scientific) equipped with a K3 camera (Gatan), a Cs corrector and a BioQuantum imaging filter (Gatan) in super-resolution mode (nominal pixel size of 0.43 A/px). Images were recorded using SerialEM over a nominal defocus range of -0.8 μm to -2.2 μm and 20 eV energy filter slit width. Collection was performed with an electron dose of 25 electrons/px/s (at the camera) in dose-fractionation mode of 0.04 seconds over a 1.4 second exposure (35 frames) to give a total dose of 47 electrons/A^2.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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4. Unaligned multi-frame micrographs of E. coli RNA polymerase on T7A1 promoter DNA with ATP, UTP, CTP - dataset 4

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6372

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.43, 0.43)

Details:

The micrographs contain unaligned raw frames. Data was collected on a 300 kV Titan Krios (ThermoFisher Scientific) equipped with a K3 camera (Gatan), a Cs corrector and a BioQuantum imaging filter (Gatan) in super-resolution mode (nominal pixel size of 0.43 A/px). Images were recorded using SerialEM over a nominal defocus range of -0.8 μm to -2.2 μm and 20 eV energy filter slit width. Collection was performed with an electron dose of 25 electrons/px/s (at the camera) in dose-fractionation mode of 0.04 seconds over a 1.4 second exposure (35 frames) to give a total dose of 47 electrons/A^2.

Files:

This entry cannot be downloaded from PDBj.

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We recommend using rsync, aspera, globus, etc.

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