micrographs - multiframe

TIFF

6075

(5760, 4092)

UNSIGNED BYTE

(0.79, 0.79)

This dataset comprises a total of 6,075 cryo-EM movies (.tif) and their corresponding gain reference files (.mrc) stored in the "data" folder. The data were collected using a JEOL JEM-Z320FHC operated at 300 kV, equipped with an in-column Omega energy filter and a K3 camera. Images were acquired with a pixel size of 0.79 Å/pixel, a nominal defocus range of -1.0 to -1.8 μm, and a 3 × 3 image shift pattern. The dataset is divided into two subsets based on the data collection date. The first subset in the "data/20250714" folder contains 2,376 movies, and the second in the "data/20250722" folder contains 3,699 movies. Note that the second subset includes low-quality movies that were excluded during live pre-processing; therefore, 3,592 of these 3,699 movies were used for the structural analysis. For the movie filenames, the three characters preceding the .tif extension (e.g., 001.tif) correspond to the specific beam shift position.

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