EMPIAR-13730 - Cryo-EM structure of the inhibitor-bound Vo complex from Enterococcus hirae [4239 multi-frame micrographs composed of 49 frames each in TIFF format]

EMPIAR-13730

Cryo-EM structure of the inhibitor-bound Vo complex from Enterococcus hirae [4239 multi-frame micrographs composed of 49 frames each in TIFF format]

Publication:

Na⁺-V-ATPase inhibitor curbs VRE growth and unveils Na⁺ pathway structure

Suzuki K, Goto Y, Otomo A, Shimizu K, Abe S, Moriyama K, Yasuda S, Hashimoto Y, Kurushima J, Mikuriya S, Imai FL, Adachi N, Kawasaki M, Sato Y, Ogasawara S, Iwata S, Senda T, Ikeguchi M, Tomita H, Iino R, Moriya T, Murata T

Nature structural & molecular biology 32 (2024) 450-458

PMID: 39572733

DOI: 10.1038/s41594-024-01419-y

Related EMDB entry:

EMD-37440

Deposited:

2026-04-22

Released:

2026-10-06

Last modified:

2026-10-06

Imageset size:

999.87 GB

Imageset DOI:

10.6019/EMPIAR-13730

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Cryo-EM structure of the inhibitor-bound Vo complex from Enterococcus hirae

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4239

Image size:

(4092, 5760)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Microscope: FEI TITAN Krios
- Detector: Gatan K3 BIOQUANTUM
- Acceleration voltage (kV): 300
- The pixel size in the image header (1 Å/px) is wrong. 0.83 Å/px is the calibrated value.
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (μm): 1.0–2.5
- Average electron dose per image (e-/A2): 49.0
- Please use 'gain.mrc' as a gain reference without rotation and y-flipping.

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Citations

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Burton Smith RN, Murata K. (2026)

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QwenCryoMarker: a universal post-processing framework for contamination-aware particle cleaning.

Sun Y, Zhao J, Xu N, Wang L, Ding W, Li M. (2026)

CryoMV: Structure-Prior-Guided Modeling and Real-Particle Validation of Continuous Conformational Transitions in Cryo-EM

Li F, Dong H, Tang S, Wang X, Zhang C, Ji C, Han R, Zhang F, Wan X. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

Helical reconstruction of amyloids in cryoSPARC.

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A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

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