EMPIAR-13538 - Raw data used for CS polymerase structure determination (CHSY3-CHPF) [12169 multi-frame micrographs composed of 66 frames each in TIFF format]

EMPIAR-13538

Raw data used for CS polymerase structure determination (CHSY3-CHPF) [12169 multi-frame micrographs composed of 66 frames each in TIFF format]

Publication:

Structural basis for human chondroitin sulfate chain polymerization

Dutta P, Cordeiro RL, Friedel-Arboleas M, Bourgeais M, Vallet SD, Weber M, Molinas M, Shu H, Grønset MNN, Miller RL, Boeri Erba E, Wild R

Nature communications 16 (2025)

PMID: 41298522

DOI: 10.1038/s41467-025-66787-5

Related EMDB entry:

EMD-52913

Deposited:

2026-04-24

Released:

2026-05-18

Last modified:

2026-05-18

Imageset size:

2.31 TB

Imageset DOI:

10.6019/EMPIAR-13538

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of the CHSY3-CHPF CS polymerase complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

12169

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(None, None)

Details:

Data collection was carried out at the European Synchrotron Radiation Facility (ESRF) on a 300 kV Titan Krios G4 (Thermo Fisher Scientific) transmission electron microscope (beamline CM02), equipped with a cold field emission gun, a Selectris X energy filter, and a Falcon 4i electron detector. 12,169 movies were collected with a total electron dose of 50 e−/Å2 at a magnification of 165,000 x and a raw pixel size of 0.73 Å.

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