EMPIAR-13503 - Single-particle cryo-EM dataset of Rhodobacter capsulatus cytochrome bc1 complex with 2-alanine insertion in the neck region of iron-sulphur protein (+2Ala mutant) [5166 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-13503

Single-particle cryo-EM dataset of Rhodobacter capsulatus cytochrome bc1 complex with 2-alanine insertion in the neck region of iron-sulphur protein (+2Ala mutant) [5166 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Distinct ubiquinone binding at the oxidation and reduction sites of cytochrome bc<sub>1</sub>

Pietras R, Wójcik-Augustyn A, Mielecki B, Sarewicz M, Jaciuk M, Koziej Ł, Glatt S, Osyczka A

Proceedings of the National Academy of Sciences of the United States of America 123 (2026)

PMID: 42679026

DOI: 10.1073/pnas.2618242123

Related EMDB entry:

EMD-57445

Deposited:

2026-04-19

Released:

2026-09-14

Last modified:

2026-09-14

Imageset size:

1.75 TB

Imageset DOI:

10.6019/EMPIAR-13503

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned dose-fractionated multi-frame micrographs of the +2Ala mutant of cytochrome bc1 complex from Rhodobacter capsulatus

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5166

Image size:

(5760, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8456, 0.8456)

Details:

---

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

Post-acquisition super resolution for cryo-electron microscopy.

Burton Smith RN, Murata K. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

Helical reconstruction of amyloids in cryoSPARC.

Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)