CryoEM movies of Rabbit muscle Aldolase determined using the SPT Labtech chameleon in the presence of 0x SurfACT. [multiple data sets in EER and MRC formats]
Publication:
A Surfactant Cocktail Overcomes Air-Water Interface Artifacts in Single-Particle CryoEM
Unaligned multi-frame micrographs of Rabbit muscle Aldolase determined using the SPT Labtech chameleon in the presence of 0x SurfACT collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2.
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Aligned micrographs of Rabbit muscle Aldolase determined using the SPT Labtech chameleon in the presence of 0x SurfACT collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were aligned in cryoSPARC.
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Gain file required for motion correction of unaligned micrographs of Rabbit muscle Aldolase determined using the SPT Labtech chameleon in the presence of 0x SurfACT.
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