EMPIAR-13389 - Cryo-EM micrographs of phosphorylated AP2 bound to the endocytic regulator NECAP [multiple data sets in TIFF format]

EMPIAR-13389

Cryo-EM micrographs of phosphorylated AP2 bound to the endocytic regulator NECAP [multiple data sets in TIFF format]

Publication:

A structural mechanism for phosphorylation-dependent inactivation of the AP2 complex

Partlow EA, Baker RW, Leschziner AE, Hollopeter G

eLife 8 (2019)

PMID: 31464684

DOI: 10.7554/elife.50003

Related EMDB entry:

EMD-20215 EMD-20220

Deposited:

2026-03-18

Released:

2026-04-08

Last modified:

2026-04-08

Imageset size:

2.59 TB

Imageset DOI:

10.6019/EMPIAR-13389

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw, unaligned cryo-EM micrographs of phosphorylated AP-2

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

542

Image size:

(7676, 7420)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.58, 0.58)

Details:

Raw, unaligned cryo-EM movies of phosphorylated AP-2 (pAP2). Movies were collected on a Talos Arctica cryoTEM with a Gatan K2 camera operating in super-resolution mode.

200 keV Talos Arctica cryoTEM
Images were collected at 36,000x, yielding a final pixel size of 1.16 Å for counting mode and 0.58 Å for super resolution mode
exposure rate of ~6 e-/pixel/s with 200 ms frames and a total exposure of ~50 e-/Å2
Cs - 2.7 mm
Ac - 0.1

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2. Raw, unaligned cryo-EM micrographs of phosphorylated AP-2 bound to NECAP

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2860

Image size:

(7676, 7420)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.58, 0.58)

Details:

Raw, unaligned cryo-EM movies of phosphorylated AP-2 (pAP2) bound to the endocytic regulator NECAP. Movies were collected on a Talos Arctica cryoTEM with a Gatan K2 camera operating in super-resolution mode.

200 keV Talos Arctica cryoTEM
Images were collected at 36,000x, yielding a final pixel size of 1.16 Å for counting mode and 0.58 Å for super resolution mode
exposure rate of ~6 e-/pixel/s with 200 ms frames and a total exposure of ~50 e-/Å2
Cs - 2.7 mm
Ac - 0.1

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Raw, unaligned cryo-EM micrographs of phosphorylated AP-2 bound to NECAP in the presence of DNA

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2314

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.16, 1.16)

Details:

Raw, unaligned cryo-EM movies of phosphorylated AP-2 (pAP2) bound to the endocytic regulator NECAP in the presence of DNA. Movies were collected on a Talos Arctica cryoTEM with a Gatan K2 camera operating in counting mode.

200 keV Talos Arctica cryoTEM
Images were collected at 36,000x, yielding a final pixel size of 1.16 Å for counting mode and 0.58 Å for super resolution mode
exposure rate of ~6 e-/pixel/s with 200 ms frames and a total exposure of ~50 e-/Å2
Cs - 2.7 mm
Ac - 0.1

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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ICECREAM: high-fidelity equivariant cryo-electron tomography.

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CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)