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EMPIAR-13355
Raw cryoEM movies for Mo-nitrogenase Av MoFeP with Gd FeP in the presence of 5 mM beryllium fluoride [multiple data sets in EER and MRC formats]
Publication:

Structural and Functional Characterization of Heterologous Nitrogenase Complexes

Li YL, Narehood SMN, Cook BDC, McGuire KLM, Herzik MAH, Tezcan FAT

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Related EMDB entry:
EMD-75568
Deposited:
2026-02-27
Released:
2026-11-09
Last modified:
2026-11-09
Imageset size:
2.03 TB
Imageset DOI:
10.6019/EMPIAR-13355
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
Image Sets
1. Unaligned multi-frame micrographs of the of Mo-nitrogenase FeP from Gluconacetobacter diazotrophicus and MoFeP from Azotobacter vinelandii in the presence of 5mM Beryllium Fluoride
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
1554
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.735, 0.735)
Details:
Unaligned multi-frame micrographs of the of Mo-nitrogenase Iron Protein (FeP) from Gluconacetobacter diazotrophicus and Molybdenum Iron Protein (MoFeP) from Azotobacter vinelandii in the presence of 5mM Beryllium Fluoride collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2. Two separate data collections that were combined are provided. Gain file included was used for correction during both collections.
Files:
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2. Gain file for motion correction of unaligned micrographs of the Mo-nitrogenase FeP from Gluconacetobacter diazotrophicus and MoFeP from Azotobacter vinelandii in the presence of 5mM Beryllium Fluoride
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
1
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.735, 0.735)
Details:
Gain file required for motion correction of unaligned micrographs of the Mo-nitrogenase Iron Protein (FeP) from Gluconacetobacter diazotrophicus and Molybdenum Iron Protein (MoFeP) from Azotobacter vinelandii in the presence of 5mM Beryllium Fluoride
Files:
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3. Unaligned multi-frame micrographs of the of Mo-nitrogenase FeP from Gluconacetobacter diazotrophicus and MoFeP from Azotobacter vinelandii in the presence of 5mM Beryllium Fluoride
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
2149
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.735, 0.735)
Details:
Unaligned multi-frame micrographs of the of Mo-nitrogenase Iron Protein (FeP) from Gluconacetobacter diazotrophicus and Molybdenum Iron Protein (MoFeP) from Azotobacter vinelandii in the presence of 5mM Beryllium Fluoride collected on a Titan Krios G4 operating at 300 kV equipped with a Selectris-X energy filter with a 10eV slit width. All images were collected at a nominal magnification of 165,000x in EF-TEM mode (with a calibrated pixel size of 0.735 Å) on a Falcon4 direct electron detector. Micrographs were acquired in EER format with a cumulative electron exposure of 60 electrons/Å2. Two separate data collections that were combined are provided. Gain file included was used for correction during both collections.
Files:
Loading...
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