EMPIAR-12978 - Single particle cryo-EM dataset of the 50S ribosomal subunit following ultrasonic excitation [4637 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12978

Single particle cryo-EM dataset of the 50S ribosomal subunit following ultrasonic excitation [4637 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ

bioRxiv (2025)

DOI: 10.1101/2025.09.14.676144

Related EMDB entry:

EMD-54829

Deposited:

2025-08-20

Released:

2026-03-23

Last modified:

2026-03-23

Imageset size:

2.63 TB

Imageset DOI:

10.6019/EMPIAR-12978

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw micrographs of 50S ribosomal subunit following ultrasonic excitation

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4637

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

---

Files:

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Citations

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

Helical reconstruction of amyloids in cryoSPARC.

Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

CryoWriter: a robotic solution for improved Cryo-EM grid preparation.

Kv C, Ekundayo B, Di Fabrizio M, Mohammed I, Radecke J, Stahlberg H, Kube M. (2026)