EMPIAR-12809 - 70S initiation complex (tRNA-fMet M1 + AUG start codon) [2267 multi-frame micrographs composed of 48 frames each in TIFF format]

EMPIAR-12809

70S initiation complex (tRNA-fMet M1 + AUG start codon) [2267 multi-frame micrographs composed of 48 frames each in TIFF format]

Publication:

Structural analysis of noncanonical translation initiation complexes

Mattingly JM, Nguyen HA, Roy B, Fredrick K, Dunham CM

Journal of Biological Chemistry 300 (2024) 107743-107743

PMID: 39222680

DOI: 10.1016/j.jbc.2024.107743

Related EMDB entry:

EMD-45573

Deposited:

2025-06-04

Released:

2025-07-30

Last modified:

2025-07-30

Imageset size:

629.29 GB

Imageset DOI:

10.6019/EMPIAR-12809

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of 70S initiation complex containing fMet-tRNA-fMet and an AUG start codon

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2267

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.045, 1.045)

Details:

Micrographs are 48-frame movies with a pixel size of 1.045 angstroms and an accumulated dose of 58.4 electrons per square angstrom. Files in the included "xml" folder contain Thermo EPU-format image shift data which can be used for beam shift group splitting. A gain reference file is included as "gain.mrc".

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