EMPIAR-12705 - Cryo-EM micrographs of TIGR-TasR in complex with tigRNA and target DNA after DNA cleavage [14257 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12705

Cryo-EM micrographs of TIGR-TasR in complex with tigRNA and target DNA after DNA cleavage [14257 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

TIGR-Tas: A family of modular RNA-guided DNA-targeting systems in prokaryotes and their viruses

Faure G, Saito M, Wilkinson ME, Quinones-Olvera N, Xu P, Flam-Shepherd D, Kim S, Reddy N, Zhu S, Evgeniou L, Koonin EV, Macrae RK, Zhang F

Science (New York, N.Y.) (2025)

PMID: 40014690

DOI: 10.1126/science.adv9789

Related EMDB entry:

EMD-48616

Deposited:

2025-04-08

Released:

2025-04-23

Last modified:

2025-04-23

Imageset size:

1.93 TB

Imageset DOI:

10.6019/EMPIAR-12705

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame movies of the TIGR-TasR RNP bound to target DNA

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

14257

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.663, 0.663)

Details:

Unaligned, non-gain-normalised, 40-frame movies from a Bioquantum-K3 camera recorded in super-resolution mode with twofold binning (within EPU) at 130,000x magnification on the MIT.nano Titan Krios G3i microscope operated at 300 keV (Cs = 2.7mm). Each micrograph accumulates 45.4 e/A2 over a 1.09s exposure time. The final refinement run_data.star file for the EMDB deposition is included as 'EMD-46818_refine_data.star', although you will need to edit the micrograph path within this file if you wish to reuse the picked coordinates. Good luck! This dataset is a little bit tricky.

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Citations

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CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

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