EMPIAR-12583 - Pichia pastoris mitochondrial complex I reconstituted into cMSP26 nanodiscs [7574 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12583

Pichia pastoris mitochondrial complex I reconstituted into cMSP26 nanodiscs [7574 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J

Science advances 11 (2025)

PMID: 41032597

DOI: 10.1126/sciadv.adz0693

Related EMDB entry:

EMD-52876 EMD-52877 EMD-52878 EMD-52875

Deposited:

2025-02-24

Released:

2025-10-30

Last modified:

2025-10-30

Imageset size:

1.02 TB

Imageset DOI:

10.6019/EMPIAR-12583

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Pichia pastoris (Komagataella phaffii) mitochondrial complex I from an as-prepared sample solubilised in LMNG and reconstituted in to cMSP26 nanodiscs.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7574

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.929, 0.929)

Details:

Unaligned multi-frame micrographs of Pichia pastoris (Komagataella phaffii) mitochondrial complex I from an as-prepared sample solubilised in LMNG and reconstituted in to cMSP26 nanodiscs. Frozen on Qunatifoil copper grid. Images (TIFF) were collected with a 300 keV FEI Titan Krios on a Falcon 4i detector, using counting mode and a SelectrisX energy filter (10 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 5.85 s exposures were captured in 40 frames with a total dose of 47.79 e-/Å-2. A pixel size of 0.929 Å/pixel was used.

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