EMPIAR-12549 - Cryo-electron micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine [multiple data sets in TIFF format]

EMPIAR-12549

Cryo-electron micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine [multiple data sets in TIFF format]

Publication:

Structural basis for catalysis and selectivity of phospholipid synthesis by eukaryotic choline-phosphotransferase

Roberts JR, Horibata Y, Kwarcinski FE, Lam V, Raczkowski AM, Hubbard A, White B, Sugimoto H, Tall GG, Ohi MD, Maeda S

Nature communications 16 (2025)

PMID: 39747155

DOI: 10.1038/s41467-024-55673-1

Related EMDB entry:

EMD-42496

Deposited:

2025-02-10

Released:

2025-07-25

Last modified:

2025-07-25

Imageset size:

4.00 TB

Imageset DOI:

10.6019/EMPIAR-12549

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 1)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3566

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.09, 1.09)

Details:

Micrographs are unaligned 60 raw movie frames accumulating a total dose of 63 electrons per square angstrom in a 3.47 second exposure.

Files:

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2. Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 2)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2563

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.08, 1.08)

Details:

Micrographs are unaligned 60 raw movie frames accumulating a total dose of 63 electrons per square angstrom in a 3.47 second exposure.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 3)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2943

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.08, 1.08)

Details:

Micrographs are unaligned 60 raw movie frames accumulating a total dose of 57 electrons per square angstrom in a 3.47 second exposure.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine with 3.25 mM CHAPS added before plunging (dataset 4)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2522

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.08, 1.08)

Details:

3.25 mM CHAPS added before plunging to ameliorate preferred orientation. Micrographs are unaligned 60 raw movie frames accumulating a total dose of 61 electrons per square angstrom in a 3.79 second exposure.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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