EMPIAR-12549
Cryo-electron micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine [multiple data sets in TIFF format]
Publication:

Structural basis for catalysis and selectivity of phospholipid synthesis by eukaryotic choline-phosphotransferase

Roberts JR, Horibata Y, Kwarcinski FE, Lam V, Raczkowski AM, Hubbard A, White B, Sugimoto H, Tall GG, Ohi MD, Maeda S

Nature communications 16 (2025)

PMID: 39747155

Related EMDB entry:
Deposited:
2025-02-10
Released:
2025-07-25
Last modified:
2025-07-25
Imageset size:
4.00 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 1)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3566
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.09, 1.09)
Details:
Micrographs are unaligned 60 raw movie frames accumulating a total dose of 63 electrons per square angstrom in a 3.47 second exposure.
Files:
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2. Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 2)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2563
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.08, 1.08)
Details:
Micrographs are unaligned 60 raw movie frames accumulating a total dose of 63 electrons per square angstrom in a 3.47 second exposure.
Files:
Loading...
3. Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine (dataset 3)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2943
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.08, 1.08)
Details:
Micrographs are unaligned 60 raw movie frames accumulating a total dose of 57 electrons per square angstrom in a 3.47 second exposure.
Files:
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4. Unaligned multi-frame micrographs of Cholinephosphotransferase in complex with CDP-choline and phosphatidylcholine with 3.25 mM CHAPS added before plunging (dataset 4)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
2522
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.08, 1.08)
Details:
3.25 mM CHAPS added before plunging to ameliorate preferred orientation. Micrographs are unaligned 60 raw movie frames accumulating a total dose of 61 electrons per square angstrom in a 3.79 second exposure.
Files:
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