EMPIAR-12469 - Cryo electron tomography of Chlamydomonas reinhardtii prepared using FIB milling [multiple data sets in MRC and MRCS formats]

EMPIAR-12469

Cryo electron tomography of Chlamydomonas reinhardtii prepared using FIB milling [multiple data sets in MRC and MRCS formats]

Publication:

MPicker: Visualizing and Picking Membrane Proteins for Cryo-Electron Tomography

Li X, Yan X, Li S, Huang W, Wang H, Zhao T, Huang M, Zhou N, Shen Y

Research Square (2024)

DOI: 10.21203/rs.3.rs-4404303/v1

Related EMDB entry:

EMD-61019

Deposited:

2024-11-30

Released:

2024-12-13

Last modified:

2024-12-13

Imageset size:

35.54 GB

Imageset DOI:

10.6019/EMPIAR-12469

Experimental metadata:

Download xml json

Contains:

reconstructed volumes
tilt series

Image Sets

1. Bin3 tomograms denoised by IsoNet

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(1364, 1920)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(10.89, 10.89)

Details:

---

Files:

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2. Bin1 unaligned stacks and other files for running Relion4

Category:

tilt series

Image format:

MRCS

No. of images or tilt series:

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(3.63, 3.63)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

CryoPROS: Correcting misalignment caused by preferred orientation using AI-generated auxiliary particles.

Zhang H, Zheng D, Wu Q, Yan N, Peng H, Hu Q, Peng Y, Yan Z, Shi Z, Bao C, Hu M. (2025)

Few-shot learning for non-vitrified ice segmentation.

Vivas-Lago A, Castaño-Díez D. (2025)

Automated removal of corrupted tilts in cryo-electron tomography.

Majtner T, Turoňová B. (2025)

Probabilistic single-particle cryo-EM ab initio 3D reconstruction in SIMPLE.

Van CTS, Reboul CF, Caesar JJE, Meana-Pañeda R, Lountos GT, Deme JC, Bryant OJ, Johnson S, Piczak CT, Valkov E, Lea SM, Elmlund H. (2025)

Reanalysis of cryo-EM data reveals ALK-cytokine assemblies with both 2:1 and 2:2 stoichiometries.

Felix J, De Munck S, Bazan JF, Savvides SN. (2025)

See all citations

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