EMPIAR-12300 - Iota toxin Ib pore serine-clamp mutant [7080 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12300

Iota toxin Ib pore serine-clamp mutant [7080 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Serine clamp of Clostridium perfringens binary toxin BECb (CPILEb)-pore confers cytotoxicity and enterotoxicity

Yoshida T, Monma C, Ninomiya Y, Takiguchi S, Fujita S, Uchida Y, Sakoda N, Karginov VA, Kishikawa J, Yamada T, Tsuge H

(---)

Related EMDB entry:

EMD-64005 EMD-39544

Deposited:

2024-09-17

Released:

2025-07-31

Last modified:

2025-07-31

Imageset size:

1.37 TB

Imageset DOI:

10.6019/EMPIAR-12300

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. K3 movies for Iota toxin Ib pore serine-clamp mutant

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7080

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.675, 0.675)

Details:

Acceleration Voltage: 300 kV
Cs: 0.0938 mm (corrected by Cs corrector)
Dose per frame: 1 electron/angstrom^2
Frames: 50 frames
Energy Filter: 20 eV
Record Mode: K3 (not CDS mode)
Defocus Range: -0.6 μm to -1.8 μm.

gain.mrc as a gain reference without rotation and flipping

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Citations

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Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)