EMPIAR-12113 - Cryo-EM structure of guide RNA and target RNA bound Cas7-11 [4177 multi-frame micrographs composed of 60 frames each in TIFF format]

EMPIAR-12113

Cryo-EM structure of guide RNA and target RNA bound Cas7-11 [4177 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

Molecular mechanism of active Cas7-11 in processing CRISPR RNA and interfering target RNA

Goswami HN, Rai J, Das A, Li H

eLife 11 (2022)

PMID: 36190192

DOI: 10.7554/elife.81678

Related EMDB entry:

EMD-27138

Deposited:

2024-05-30

Released:

2024-06-13

Last modified:

2024-06-13

Imageset size:

1.98 TB

Imageset DOI:

10.6019/EMPIAR-12113

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame microgtaphs of target RNA bound active DiCas7-11-crRNA complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4177

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(None, None)

Details:

---

Files:

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Citations

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

Helical reconstruction of amyloids in cryoSPARC.

Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

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prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

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CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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