EMPIAR-11983 - Cryo electron tomography of FIB-milled E. coli strain NCM3722 grown to mid-log phase in rich media [1994 multi-frame micrographs composed of 8 frames each in TIFF format]

EMPIAR-11983

Cryo electron tomography of FIB-milled E. coli strain NCM3722 grown to mid-log phase in rich media [1994 multi-frame micrographs composed of 8 frames each in TIFF format]

Publication:

Rapid structural analysis of bacterial ribosomes in situ

Powell BM, Brant TS, Davis JH, Mosalaganti S

Communications biology 8 (2025)

PMID: 39875527

DOI: 10.1038/s42003-025-07586-y

Related EMDB entry:

EMD-44152

Deposited:

2024-03-20

Released:

2026-11-02

Last modified:

2026-11-02

Imageset size:

46.32 GB

Imageset DOI:

10.6019/EMPIAR-11983

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Tilt series unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1994

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.368, 1.368)

Details:

Tilt series were acquired on a Titan Krios G4i microscope (ThermoFisher Scientific) operated at 300 kV and equipped with a Bioquantum energy filter (Gatan). Movies were collected at a magnification of 64,000x with a K3 Summit direct electron detector (Gatan) operating in super-resolution mode and binned 2x, corresponding to a nominal physical pixel size of 1.368 Å, with 8 frames per movie. Tilts were collected using SerialEM software with a modified dose-symmetric tilt scheme ranging from -54 to +66 degrees, starting from a +6° pretilt and at an interval of 3°. The total dose per tilt series was 100 e-/Å2. A 20 eV energy filter slit was used in the record mode, and the energy filter Zero Loss Peak was refined after each tilt series acquisition.

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