EMPIAR-11931 - Cryo electron structure of the ectodomain of Site-one protease without SPRING [6171 multi-frame micrographs composed of 1 frames each in EER format]

EMPIAR-11931

Cryo electron structure of the ectodomain of Site-one protease without SPRING [6171 multi-frame micrographs composed of 1 frames each in EER format]

Publication:

SPRING licenses S1P-mediated cleavage of SREBP2 by displacing an inhibitory pro-domain

Hendrix S, Dartigue V, Hall H, Bawaria S, Kingma J, Bajaj B, Zelcer N, Kober DL

Nature communications 15 (2024)

PMID: 38977690

DOI: 10.1038/s41467-024-50068-8

Related EMDB entry:

EMD-42661

Deposited:

2024-03-02

Released:

2025-02-10

Last modified:

2025-02-10

Imageset size:

3.06 TB

Imageset DOI:

10.6019/EMPIAR-11931

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. eer format movies of site-one protease

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

6171

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.738, 0.738)

Details:

Unprocessed micrographs and gain ref file for data collection corresponding to EMD-42661. The pixel size was 0.738 A. The nominal dose rate was 8.5 eps and the total dose was 55 e/A2. For processing, the movies were split into 50 frames.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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