EMPIAR-11836 - Cryo-EM of BtCoV-HKU5 5' proximal stem-loop 5 [multiple data sets in TIFF and MRC formats]

EMPIAR-11836

Cryo-EM of BtCoV-HKU5 5' proximal stem-loop 5 [multiple data sets in TIFF and MRC formats]

Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

DOI: 10.1073/pnas.2320493121

Related EMDB entry:

EMD-42801 EMD-42805 EMD-42808 EMD-42802

Deposited:

2023-11-28

Released:

2024-04-07

Last modified:

2024-04-07

Imageset size:

12.86 TB

Imageset DOI:

10.6019/EMPIAR-11836

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (exposures*/*/FoilHole_*_Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   23,775,774,596 kilobytes
Compressed size: 13,659,721,181 kilobytes

Contains:

micrographs - multiframe
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame micrographs of BtCoV-HKU5 5' stem-loops 5 RNA dataset 1.

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5728

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.82, 0.82)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 49.97 electrons per angstrom squared with a total exposure time of 4.74 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are gain corrected.

Files:

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2. Extracted particles and orientations for final reconstruction of SARS-CoV-2 5' stem-loops 5-6 RNA.

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

666400

Image size:

(200, 200)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.82, 0.82)

Details:

Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/BtCoV-HKU5_SL5/particles/conformation*.cs for each of the 4 conformations.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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3. Unaligned multi-frame micrographs of BtCoV-HKU5 5' stem-loops 5 RNA dataset 2.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

12411

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.82, 0.82)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 49.79 electrons per angstrom squared with a total exposure time of 3.81 seconds. Desired defocuses ranged from -1.2 to -2.2 nm. The exposures are gain corrected.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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