EMPIAR-11836
Cryo-EM of BtCoV-HKU5 5' proximal stem-loop 5 [multiple data sets in TIFF and MRC formats]
Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

Related EMDB entry:
Deposited:
2023-11-28
Released:
2024-04-07
Last modified:
2024-04-07
Imageset size:
22.28 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • picked particles - single frame - processed
1. Unaligned multi-frame micrographs of BtCoV-HKU5 5' stem-loops 5 RNA dataset 1.
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5728
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.82, 0.82)
Details:
These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 49.97 electrons per angstrom squared with a total exposure time of 4.74 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are gain corrected.
Files:
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2. Extracted particles and orientations for final reconstruction of SARS-CoV-2 5' stem-loops 5-6 RNA.
Category:
picked particles - single frame - processed
Image format:
MRC
No. of images or tilt series:
666400
Image size:
(200, 200)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.82, 0.82)
Details:
Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/BtCoV-HKU5_SL5/particles/conformation*.cs for each of the 4 conformations.
Files:
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3. Unaligned multi-frame micrographs of BtCoV-HKU5 5' stem-loops 5 RNA dataset 2.
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
12411
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.82, 0.82)
Details:
These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector. Over the collection, the sample was exposed evenly to 49.79 electrons per angstrom squared with a total exposure time of 3.81 seconds. Desired defocuses ranged from -1.2 to -2.2 nm. The exposures are gain corrected.
Files:
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Files:
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