EMPIAR-11834
Cryo-EM of SARS-CoV-2 5' proximal stem-loops 5-6 with SL5b extended [multiple data sets in MRC format]
Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

Related EMDB entry:
Deposited:
2023-11-28
Released:
2024-04-17
Last modified:
2024-04-17
Imageset size:
14.82 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • picked particles - single frame - processed
  • micrographs - multiframe
1. Extracted particles and orientations for final reconstruction of SARS-CoV-2 5' stem-loops 5-6 RNA with SL5b extended.
Category:
picked particles - single frame - processed
Image format:
MRC
No. of images or tilt series:
199745
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.741, 0.741)
Details:
Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/SARS-CoV-2_SL5-6_5bext/particles/P3_J1305_particles_exported.cs.
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2. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5-6 RNA with SL5b extended.
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
11496
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.741, 0.741)
Details:
These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector and a Selectris energy filter with 10eV slit. Over the collection, the sample was exposed evenly to 50.12 electrons per angstrom squared with a total exposure time of 4.66 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are gain corrected.
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