EMPIAR-11834 - Cryo-EM of SARS-CoV-2 5' proximal stem-loops 5-6 with SL5b extended [multiple data sets in MRC format]

EMPIAR-11834

Cryo-EM of SARS-CoV-2 5' proximal stem-loops 5-6 with SL5b extended [multiple data sets in MRC format]

Publication:

Tertiary folds of the SL5 RNA from the 5' proximal region of SARS-CoV-2 and related coronaviruses

Kretsch RC, Xu L, Zheludev IN, Zhou X, Huang R, Nye G, Li S, Zhang K, Chiu W, Das R

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 38427602

DOI: 10.1073/pnas.2320493121

Related EMDB entry:

EMD-42820

Deposited:

2023-11-28

Released:

2024-04-17

Last modified:

2024-04-17

Imageset size:

14.82 TB

Imageset DOI:

10.6019/EMPIAR-11834

Experimental metadata:

Download xml json

Contains:

picked particles - single frame - processed
micrographs - multiframe

Image Sets

1. Extracted particles and orientations for final reconstruction of SARS-CoV-2 5' stem-loops 5-6 RNA with SL5b extended.

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

199745

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.741, 0.741)

Details:

Particles extracted from motion-corrected micrographs. The orientations for the final reconstruction can be found in data/SARS-CoV-2_SL5-6_5bext/particles/P3_J1305_particles_exported.cs.

Files:

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2. Unaligned multi-frame micrographs of SARS-CoV-2 5' stem-loops 5-6 RNA with SL5b extended.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

11496

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.741, 0.741)

Details:

These micrographs were collected with a Titan Krios G3i, 300kV and a Falcon 4 detector and a Selectris energy filter with 10eV slit. Over the collection, the sample was exposed evenly to 50.12 electrons per angstrom squared with a total exposure time of 4.66 seconds. Desired defocuses ranged from -1 to -2 nm. The exposures are gain corrected.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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