EMPIAR-11791 - Single-Particle Cryo-EM of AAV2 at Various Tilts [multiple data sets in MRCS and MRC formats]

EMPIAR-11791

Single-Particle Cryo-EM of AAV2 at Various Tilts [multiple data sets in MRCS and MRC formats]

Publication:

Overcoming resolution attenuation during tilted cryo-EM data collection

Aiyer S, Baldwin PR, Tan SM, Shan Z, Oh J, Mehrani A, Bowman ME, Louie G, Passos DO, Đorđević-Marquardt S, Mietzsch M, Hull JA, Hoshika S, Barad BA, Grotjahn DA, McKenna R, Agbandje-McKenna M, Benner SA, Noel JAP, Wang D, Tan YZ, Lyumkis D

Nature communications 15 (2024)

PMID: 38195598

DOI: 10.1038/s41467-023-44555-7

Related EMDB entry:

EMD-36768 EMD-36766 EMD-36769 EMD-36772 EMD-36770 EMD-36771 EMD-36767

Deposited:

2023-11-23

Released:

2024-02-13

Last modified:

2024-02-13

Imageset size:

730.42 GB

Imageset DOI:

10.6019/EMPIAR-11791

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - multiframe - processed

Image Sets

1. Unaligned multiframe movies of AAV2 collected at various tilts

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

1229

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.026, 1.026)

Details:

Movies collected using Titan Krios and K2 summit. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders. 200 frames were used, pixel size is 1.026 Å/pixel, total dose of 78.0 e-/Å2. Compressed using pbzip2. Gain reference is 17jun01a_01155404_17_3838x3710_norm_0.mrc and needs to be flipped upside down to use.

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2. Aligned and dose-weighted micrographs of AAV2 collected at various tilts

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1229

Image size:

(3710, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.026, 1.026)

Details:

Aligned and dose weighted using UCSF MotionCor2. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders.

Files:

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3. Polished and equalized particles of AAV2 collected at various tilts

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(600, 600)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.026, 1.026)

Details:

Final particle stack after particle polishing using Relion. The particles are equalized so that there are same number of particles per tilt angle with similar defocus distribution.

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Browse All Files

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