EMPIAR-11784
SpCas9 bound to 18 nucleotide complementary DNA substrate in the checkpoint state [multiple data sets in EER format]
Publication:

R-loop formation and conformational activation mechanisms of Cas9

Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M

Nature 609 (2022) 191-196

PMID: 36002571

Related EMDB entry:
Deposited:
2023-11-10
Released:
2024-02-06
Last modified:
2024-02-06
Imageset size:
8.49 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
8541
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.45, 0.45)
Details:
Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.
Files:
Loading...
2. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
8199
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.45, 0.45)
Details:
Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.
Files:
Loading...
3. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
7344
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.45, 0.45)
Details:
Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.
Files:
Loading...
4. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
7890
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.45, 0.45)
Details:
Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.
Files:
Loading...
5. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
8541
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.45, 0.45)
Details:
Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.
Files:
Loading...