EMPIAR-11784
SpCas9 bound to 18 nucleotide complementary DNA substrate in the checkpoint state [multiple data sets in EER format]
Publication:

R-loop formation and conformational activation mechanisms of Cas9

Pacesa M, Loeff L, Querques I, Muckenfuss LM, Sawicka M, Jinek M

Nature 609 (2022) 191-196

PMID: 36002571

Related EMDB entry:
Deposited:
2023-11-10
Released:
2024-02-06
Last modified:
2024-02-06
Imageset size:
7.72 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
8541
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.45, 0.45)
Details:
Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.
Files:
Loading...
2. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
8199
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.45, 0.45)
Details:
Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.
Files:
Loading...
3. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
7344
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.45, 0.45)
Details:
Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.
Files:
Loading...
4. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
7890
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.45, 0.45)
Details:
Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.
Files:
Loading...
5. Unaligned multi-frame micrographs of SpCas9 bound to 18 nucleotide complementary DNA substrate in checkpoint state
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
8541
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.45, 0.45)
Details:
Unaligned multi-frame movies with each containing 532 frames per movie collected at 300 kV on FALCON 4 detector. Total accumulated electron exposure on the specimen level corresponds to 132.9 electrons per Å^2.
Files:
Loading...
Files:
Loading...
Citations

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)