EMPIAR-11687 - Cryo electron tomography of Cytochalasin D-induced protrusions of Drosophila S2 cells treated with thapsigargin or MG132 [multiple data sets in TIFF and MRC formats]

EMPIAR-11687

Cryo electron tomography of Cytochalasin D-induced protrusions of Drosophila S2 cells treated with thapsigargin or MG132 [multiple data sets in TIFF and MRC formats]

Publication:

CryoET shows cofilactin filaments inside the microtubule lumen

Ventura Santos C, Carter AP, Rogers SL

EMBO Reports (2023)

PMID: 37702953

DOI: 10.15252/embr.202357264

Related EMDB entry:

EMD-18475

Deposited:

2023-09-18

Released:

2023-10-03

Last modified:

2023-10-03

Imageset size:

107.59 GB

Imageset DOI:

10.6019/EMPIAR-11687

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
reconstructed volumes

Image Sets

1. Raw multi-frame tilt images of Cytochalasin D and thapsigargin or MG132 treated protrusions of Drosophila S2 cells

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.329, 1.329)

Details:

This folder contains raw, multi-frame tilt images. Data was collected on a K3 detector in super-resolution mode with a super-resolution pixel size of 1.329Ã…/pixel. Mdoc files and the gain reference are provided inside the folder.
This data is from the preliminary experiment shown in Fig. EV2A of the associated publication. TS_167, TS_169, TS_171, TS_172, TS_173 are from cells treated with 2.5uM Cytochalasin D (2h) and 25uM MG132 (5h).
TS_178, TS_179, TS_180, TS_181, TS_182, TS_183, TS_185, TS_186 are from cells treated with 2.5uM Cytochalasin D (2h) and 2uM thapsigargin (5h).

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2. Binned, deconvolved tomograms of Cytochalasin D and thapsigargin or MG132 treated protrusions of Drosophila S2 cells

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(1022, 1440)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(10.74, 10.74)

Details:

Tomograms binned by 4 (from non-super resolution pixel size), reconstructed and deconvolved in WARP.
This data is from the preliminary experiment shown in Fig. EV2A of the associated publication. TS_167, TS_169, TS_171, TS_172, TS_173 are from cells treated with 2.5uM Cytochalasin D (2h) and 25uM MG132 (5h).
TS_178, TS_179, TS_180, TS_181, TS_182, TS_183, TS_185, TS_186 are from cells treated with 2.5uM Cytochalasin D (2h) and 2uM thapsigargin (5h).

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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