EMPIAR-11371 - Cryogenic electron tomographs of Coxsackievirus A9 treated with fatty-acid-free BSA [2472 multi-frame micrographs composed of 30 frames each in MRC format]

EMPIAR-11371

Cryogenic electron tomographs of Coxsackievirus A9 treated with fatty-acid-free BSA [2472 multi-frame micrographs composed of 30 frames each in MRC format]

Publication:

Structural Studies Reveal that Endosomal Cations Promote Formation of Infectious Coxsackievirus A9 A-Particles, Facilitating RNA and VP4 Release

Domanska A, Plavec Z, Ruokolainen V, Löflund B, Marjomäki V, Butcher SJ

Journal of virology 96 (2022)

PMID: 36448797

DOI: 10.1128/jvi.01367-22

Related EMDB entry:

EMD-15692

Deposited:

2023-01-05

Released:

2023-01-19

Last modified:

2023-01-19

Imageset size:

2.26 TB

Imageset DOI:

10.6019/EMPIAR-11371

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw micrographs of CVA9 treated with fatty-acid free BSA for 1h

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2472

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.97, 0.97)

Details:

Micrographs were collected on FEI Talos Arctica operating at 200 kV with a Falcon III direct electron detector at a nominal magnification of ×150,000. Each movie consisted of 30 frames with an exposure time of 1 s per frame. The total electron dose was approximately 30 electrons per Å2.

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Citations

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He L, Bartesaghi A. (2026)

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Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

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ICECREAM: high-fidelity equivariant cryo-electron tomography.

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Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)