EMPIAR-11336 - Cryo-EM SPA datasets for Antibody 4-33 in complex with prefusion SARS-CoV-2 Spike glycoprotein (K3 movies/.tif files) [6976 multi-frame micrographs composed of 60 frames each in TIFF format]

EMPIAR-11336

Cryo-EM SPA datasets for Antibody 4-33 in complex with prefusion SARS-CoV-2 Spike glycoprotein (K3 movies/.tif files) [6976 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

Antibodies targeting a quaternary site on SARS-CoV-2 spike glycoprotein prevent viral receptor engagement by conformational locking

Liu L, Casner RG, Guo Y, Wang Q, Iketani S, Chan JF, Yu J, Dadonaite B, Nair MS, Mohri H, Reddem ER, Yuan S, Poon VK, Chan CC, Yuen KY, Sheng Z, Huang Y, Bloom JD, Shapiro L, Ho DD

Immunity (2023)

PMID: 37776849

DOI: 10.1016/j.immuni.2023.09.003

Related EMDB entry:

EMD-26964

Deposited:

2022-12-03

Released:

2023-05-26

Last modified:

2023-10-09

Imageset size:

1.80 TB

Imageset DOI:

10.6019/EMPIAR-11336

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. K3 Movies (.tif) for 4-33 Antibody + Spike

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6976

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.07, 1.07)

Details:

Collected on the Columbia Zuckerman Institute Krios using K3-BioQuantum.
Dose: 16 e/pix/s, Pixel Size: 1.074 A/pix, Exposure time 3s, Frame time 0.05s, Total Dose 42e/A2

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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