EMPIAR-11336 - Cryo-EM SPA datasets for Antibody 4-33 in complex with prefusion SARS-CoV-2 Spike glycoprotein (K3 movies/.tif files) [6976 multi-frame micrographs composed of 60 frames each in TIFF format]

EMPIAR-11336

Cryo-EM SPA datasets for Antibody 4-33 in complex with prefusion SARS-CoV-2 Spike glycoprotein (K3 movies/.tif files) [6976 multi-frame micrographs composed of 60 frames each in TIFF format]

Publication:

Antibodies targeting a quaternary site on SARS-CoV-2 spike glycoprotein prevent viral receptor engagement by conformational locking

Liu L, Casner RG, Guo Y, Wang Q, Iketani S, Chan JF, Yu J, Dadonaite B, Nair MS, Mohri H, Reddem ER, Yuan S, Poon VK, Chan CC, Yuen KY, Sheng Z, Huang Y, Bloom JD, Shapiro L, Ho DD

Immunity (2023)

PMID: 37776849

DOI: 10.1016/j.immuni.2023.09.003

Related EMDB entry:

EMD-26964

Deposited:

2022-12-03

Released:

2023-05-26

Last modified:

2023-10-09

Imageset size:

1.80 TB

Imageset DOI:

10.6019/EMPIAR-11336

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. K3 Movies (.tif) for 4-33 Antibody + Spike

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6976

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.07, 1.07)

Details:

Collected on the Columbia Zuckerman Institute Krios using K3-BioQuantum.
Dose: 16 e/pix/s, Pixel Size: 1.074 A/pix, Exposure time 3s, Frame time 0.05s, Total Dose 42e/A2

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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