EMPIAR-11312 - LRRC8A-BRIL:C Heteromer in lipid nanodiscs [multiple data sets in TIFF and MRCS formats]

EMPIAR-11312

LRRC8A-BRIL:C Heteromer in lipid nanodiscs [multiple data sets in TIFF and MRCS formats]

Publication:

Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels

Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG

bioRxiv (2022)

PMID: 30775971

DOI: 10.1101/2022.07.31.502239

Related EMDB entry:

EMD-27686 EMD-27687

Deposited:

2022-08-15

Released:

2023-03-14

Last modified:

2023-03-14

Imageset size:

2.42 TB

Imageset DOI:

10.6019/EMPIAR-11312

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - multiframe - processed

Image Sets

1. Unaligned tif movies for LRRC8ABRIL:C in lipid nanodiscs

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3906

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.524, 0.524)

Details:

Gain reference in dm4 and mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 0.9948 e/Å2 dose per frame and use bin 2 to produce mrc at the physical pixel size of 1.048Å/px.

Files:

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2. Polished particles stacks for LRRC8A-BRIL/C in lipid nanodiscs

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

3581

Image size:

(416, 416)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.048, 1.048)

Details:

Contains polished particles (192,578) as 3581 mrcs files. The selected particles (176,166) for the final reconstruction are included in the main data folder. They are in RELION star format and can be imported into cryoSPARC as well for processing.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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