EMPIAR-11312
LRRC8A-BRIL:C Heteromer in lipid nanodiscs [multiple data sets in TIFF and MRCS formats]
Publication:

Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels

Kern DM, Bleier J, Mukherjee S, Hill JM, Kossiakoff AA, Isacoff EY, Brohawn SG

bioRxiv (2022)

PMID: 30775971

Related EMDB entry:
Deposited:
2022-08-15
Released:
2023-03-14
Last modified:
2023-03-14
Imageset size:
2.42 TB
Imageset DOI:
Experimental metadata:
Contains:
  • micrographs - multiframe
  • picked particles - multiframe - processed
1. Unaligned tif movies for LRRC8ABRIL:C in lipid nanodiscs
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
3906
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.524, 0.524)
Details:
Gain reference in dm4 and mrc form is included in the folder with the movies. In RELION, the gain must be flipped upside down. To match our motion correction settings, use 0.9948 e/Å2 dose per frame and use bin 2 to produce mrc at the physical pixel size of 1.048Å/px.
Files:
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2. Polished particles stacks for LRRC8A-BRIL/C in lipid nanodiscs
Category:
picked particles - multiframe - processed
Image format:
MRCS
No. of images or tilt series:
3581
Image size:
(416, 416)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.048, 1.048)
Details:
Contains polished particles (192,578) as 3581 mrcs files. The selected particles (176,166) for the final reconstruction are included in the main data folder. They are in RELION star format and can be imported into cryoSPARC as well for processing.
Files:
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Files:
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Citations
Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)
See all citations
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