EMPIAR-11235 - Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore [11284 multi-frame micrographs composed of 84 frames each in TIFF format]

EMPIAR-11235

Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore [11284 multi-frame micrographs composed of 84 frames each in TIFF format]

Publication:

Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore from Clostridioides difficile

Kawamoto A, Yamada T, Yoshida T, Sato Y, Kato T, Tsuge H

Nature communications 13 (2022)

PMID: 36253419

DOI: 10.1038/s41467-022-33888-4

Related EMDB entry:

EMD-32043 EMD-32041

Deposited:

2022-09-22

Released:

2022-11-14

Last modified:

2022-11-14

Imageset size:

3.27 TB

Imageset DOI:

10.6019/EMPIAR-11235

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. K3 movies for CDTa-bound CDTb-pore complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11284

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.88, 0.88)

Details:

Acceleration Voltage: 300 kV
Cs: 0.01 mm (corrected by Cs corrector)
Dose per frame: 0.5952 electron/angstrom^2
Frames: 84 frames
Energy Filter: 20 eV
Record Mode: K3 (not CDS mode)
Defocus Range: -0.8 μm to -2.0 μm.

gain.mrc as a gain reference without rotation and flipping

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