EMPIAR-11235
Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore [11284 multi-frame micrographs composed of 84 frames each in TIFF format]
Publication:

Cryo-EM structures of the translocational binary toxin complex CDTa-bound CDTb-pore from Clostridioides difficile

Kawamoto A, Yamada T, Yoshida T, Sato Y, Kato T, Tsuge H

Nature communications 13 (2022)

PMID: 36253419

Related EMDB entry:
Deposited:
2022-09-22
Released:
2022-11-14
Last modified:
2022-11-14
Imageset size:
3.27 TB
Imageset DOI:
Experimental metadata:
Download xml json
Deposited via PDBj
Contains:
  • micrographs - multiframe
1. K3 movies for CDTa-bound CDTb-pore complex
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
11284
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.88, 0.88)
Details:
Acceleration Voltage: 300 kV
Cs: 0.01 mm (corrected by Cs corrector)
Dose per frame: 0.5952 electron/angstrom^2
Frames: 84 frames
Energy Filter: 20 eV
Record Mode: K3 (not CDS mode)
Defocus Range: -0.8 μm to -2.0 μm.

gain.mrc as a gain reference without rotation and flipping
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